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puno1 mil2  (InvivoGen)


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    InvivoGen puno1 mil2
    Puno1 Mil2, supplied by InvivoGen, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/puno1+mil2/pUNO1-mIL2/pm41592890-58-20-44
    Average 92 stars, based on 2 article reviews
    puno1 mil2 - by Bioz Stars, 2026-10
    92/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Reprogramming the melanoma tumor immune microenvironment via combinatorial signal 2/3 gene delivery
    Article Snippet: .. A pcDNA3 plasmid encoding firefly luciferase was obtained from Elim Biopharmaceuticals. pUNO1-mIL2, pUNO1-mIL12, pUNO1-mIL15, pUNO1-mIL23, pUNO1-mIL35, pUNO1-mGMCSF, pUNO1-m41BBL, pUNO1-mCD80, pUNO1-mCD86, and pUNO1-mOX40L were obtained from Invivogen. ..

    Article Title: Reprogramming the melanoma tumor immune microenvironment via combinatorial signal 2/3 gene delivery.
    Article Snippet: .. Expression of costimulatory molecules and immunostimulatory cytokines in vitro A pcDNA3 plasmid encoding firefly luciferase was obtained from Elim Biopharmaceuticals. pUNO1- mIL2, pUNO1- mIL12, pUNO1- mIL15, pUNO1- mIL23, pUNO1- mIL35, pUNO1- mGMCSF, pUNO1- m41BBL, pUNO1- mCD80, pUNO1- mCD86, and pUNO1- mOX40L were obtained from Invivogen. ..

    Luciferase:

    Article Title: Reprogramming the melanoma tumor immune microenvironment via combinatorial signal 2/3 gene delivery
    Article Snippet: .. A pcDNA3 plasmid encoding firefly luciferase was obtained from Elim Biopharmaceuticals. pUNO1-mIL2, pUNO1-mIL12, pUNO1-mIL15, pUNO1-mIL23, pUNO1-mIL35, pUNO1-mGMCSF, pUNO1-m41BBL, pUNO1-mCD80, pUNO1-mCD86, and pUNO1-mOX40L were obtained from Invivogen. ..

    Article Title: Reprogramming the melanoma tumor immune microenvironment via combinatorial signal 2/3 gene delivery.
    Article Snippet: .. Expression of costimulatory molecules and immunostimulatory cytokines in vitro A pcDNA3 plasmid encoding firefly luciferase was obtained from Elim Biopharmaceuticals. pUNO1- mIL2, pUNO1- mIL12, pUNO1- mIL15, pUNO1- mIL23, pUNO1- mIL35, pUNO1- mGMCSF, pUNO1- m41BBL, pUNO1- mCD80, pUNO1- mCD86, and pUNO1- mOX40L were obtained from Invivogen. ..

    Expressing:

    Article Title: Reprogramming the melanoma tumor immune microenvironment via combinatorial signal 2/3 gene delivery.
    Article Snippet: .. Expression of costimulatory molecules and immunostimulatory cytokines in vitro A pcDNA3 plasmid encoding firefly luciferase was obtained from Elim Biopharmaceuticals. pUNO1- mIL2, pUNO1- mIL12, pUNO1- mIL15, pUNO1- mIL23, pUNO1- mIL35, pUNO1- mGMCSF, pUNO1- m41BBL, pUNO1- mCD80, pUNO1- mCD86, and pUNO1- mOX40L were obtained from Invivogen. ..

    In Vitro:

    Article Title: Reprogramming the melanoma tumor immune microenvironment via combinatorial signal 2/3 gene delivery.
    Article Snippet: .. Expression of costimulatory molecules and immunostimulatory cytokines in vitro A pcDNA3 plasmid encoding firefly luciferase was obtained from Elim Biopharmaceuticals. pUNO1- mIL2, pUNO1- mIL12, pUNO1- mIL15, pUNO1- mIL23, pUNO1- mIL35, pUNO1- mGMCSF, pUNO1- m41BBL, pUNO1- mCD80, pUNO1- mCD86, and pUNO1- mOX40L were obtained from Invivogen. ..



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    Development of IL-27 chimeric cytokines. ( A ) Generation 1 (Gen1) of IL-27 (IL27p28 and EBI3 subunits) with pepL at the C-terminus. Antitumor pathways listed briefly summarize the signaling effects reported in . ( B ) Generation 2 (Gen2) of the IL27.pepL consists of its fusion at the N-terminus to a secreted Nanoluc (Nluc). Shown is the vector evolution of targeted Nanoluc-27 (Nluc-27.pepL). Mouse IL-27elasti fragment from <t>pUNO1-mIL-27(ebi3p28)</t> (InvivoGen) with a 3′ insertion of a sequence-encoding peptide linker (GGGGS) (not shown) followed by the sequence for the targeting peptide (pepL: LSLITRL), lime green; scrambled control (scr1: IRSLTLL), not shown; or irrelevant-peptide control (irrel1: SIFSSHM), not shown. pNLF1-secNluc (Promega) was used as the vector backbone and was linearized using EcoRI. NEBuilders HiFi DNA-assembly cloning kit (#E5520S) was used to generate plasmids with the fusions of Nluc and targeted IL-27-expression vectors. ( C ) In silico modeling of the final construct of Nluc-27 (I-TASSER) showing the targeting module (pepL) is solvent exposed (lime green).
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    Development of IL-27 chimeric cytokines. ( A ) Generation 1 (Gen1) of IL-27 (IL27p28 and EBI3 subunits) with pepL at the C-terminus. Antitumor pathways listed briefly summarize the signaling effects reported in . ( B ) Generation 2 (Gen2) of the IL27.pepL consists of its fusion at the N-terminus to a secreted Nanoluc (Nluc). Shown is the vector evolution of targeted Nanoluc-27 (Nluc-27.pepL). Mouse IL-27elasti fragment from pUNO1-mIL-27(ebi3p28) (InvivoGen) with a 3′ insertion of a sequence-encoding peptide linker (GGGGS) (not shown) followed by the sequence for the targeting peptide (pepL: LSLITRL), lime green; scrambled control (scr1: IRSLTLL), not shown; or irrelevant-peptide control (irrel1: SIFSSHM), not shown. pNLF1-secNluc (Promega) was used as the vector backbone and was linearized using EcoRI. NEBuilders HiFi DNA-assembly cloning kit (#E5520S) was used to generate plasmids with the fusions of Nluc and targeted IL-27-expression vectors. ( C ) In silico modeling of the final construct of Nluc-27 (I-TASSER) showing the targeting module (pepL) is solvent exposed (lime green).

    Journal: Bioengineering

    Article Title: A Second-Generation Nanoluc-IL27 Fusion Cytokine for Targeted-Gene-Therapy Applications

    doi: 10.3390/bioengineering9020077

    Figure Lengend Snippet: Development of IL-27 chimeric cytokines. ( A ) Generation 1 (Gen1) of IL-27 (IL27p28 and EBI3 subunits) with pepL at the C-terminus. Antitumor pathways listed briefly summarize the signaling effects reported in . ( B ) Generation 2 (Gen2) of the IL27.pepL consists of its fusion at the N-terminus to a secreted Nanoluc (Nluc). Shown is the vector evolution of targeted Nanoluc-27 (Nluc-27.pepL). Mouse IL-27elasti fragment from pUNO1-mIL-27(ebi3p28) (InvivoGen) with a 3′ insertion of a sequence-encoding peptide linker (GGGGS) (not shown) followed by the sequence for the targeting peptide (pepL: LSLITRL), lime green; scrambled control (scr1: IRSLTLL), not shown; or irrelevant-peptide control (irrel1: SIFSSHM), not shown. pNLF1-secNluc (Promega) was used as the vector backbone and was linearized using EcoRI. NEBuilders HiFi DNA-assembly cloning kit (#E5520S) was used to generate plasmids with the fusions of Nluc and targeted IL-27-expression vectors. ( C ) In silico modeling of the final construct of Nluc-27 (I-TASSER) showing the targeting module (pepL) is solvent exposed (lime green).

    Article Snippet: PCR cloning was utilized to clone the mouse IL-27elasti fragment from pUNO1-mIL-27(ebi3p28) (InvivoGen.

    Techniques: Plasmid Preparation, Sequencing, Clone Assay, Expressing, In Silico, Construct, Solvent